development of a freeze-dried radiopharmaceutical kit for dopamine transporters imaging
نویسندگان
چکیده
introduction: 99mtc-trodat-1 is a promising new radiopharmaceutical with the potential for routine use as the radiopharmaceutical for dopamine transporters scintigraphy as far as its image quality and daily availability are concerned. here we describe the development of a freeze-dried kit formulation based on the tricine exchange labeling approach for the preparation of this radiopharmaceutical in a clinical setting. methods: a freeze-dried formulation contained of trodat-1, tricine, sncl2 and manitol was prepared. labeling was performed by addition of 1480 mbq 99mtc sodium pertechnetate in a total volume of 2 ml and incubation for 15 min in a boiling water bath. radiochemical analysis involved itlc and hplc methods. the stability of radioconjugate was checked in the presence of human serum at 37 °c up to 24 h. results: 99mtc-trodat-1 was prepared with a radiochemical purity of >95% and a high stability up to 24 h of the final preparation, and retained biological activity. conclusion: the developed kit formulation forms the basis for further clinical evaluation of this promising new radiopharmaceutical.
منابع مشابه
Development of a freeze-dried radiopharmaceutical kit for dopamine transporters imaging
Introduction: 99mTc-TRODAT-1 is a promising new radiopharmaceutical with the potential for routine use as the radiopharmaceutical for dopamine transporters scintigraphy as far as its image quality and daily availability are concerned. Here we describe the development of a freeze-dried kit formulation based on the tricine exchange labeling approach for the preparation of this radiopha...
متن کاملFreeze-dried cold kit for preparation of 99mtc-ciprofloxacin as an infection imaging agent
Introduction: Radiolabeled antibiotics are being used for the specific diagnosis of infection by exploiting their specific binding properties to the bacterial components, thereby making it possible to differentiate infection from sterile lesions. The aim of this work was to prepare and evaluate a freeze-dried kit of ciprofloxacin designed for the labeling with ...
متن کاملNew peptide based freeze-dried kit [99mTc-HYNIC]-UBI 29-41 as a human specific infection imaging agent
Introduction: Ubiquicidin 29-41 (UBI) is a fragment of the cationic antimicrobial peptide that is present in various species including humans. The purpose of this study was to investigate radiochemical and biological characteristics of [6-hydrazinopyridine-3-carboxylic acid (HYNIC)]-UBI 29-41 designed for the labeling with 99mTc using tricine as coligand....
متن کاملfreeze-dried cold kit for preparation of 99mtc-ciprofloxacin as an infection imaging agent
introduction: radiolabeled antibiotics are being used for the specific diagnosis of infection by exploiting their specific binding properties to the bacterial components, thereby making it possible to differentiate infection from sterile lesions. the aim of this work was to prepare and evaluate a freeze-dried kit of ciprofloxacin designed for the labeling with 99mtc. methods: factors like amou...
متن کاملNew freeze-dried kit for diagnosis of Bombesin receptor expressing tumors
Introduction: It has been shown that some primary human tumors and their metastases, including prostate and breast tumors, over-express gastrin-releasing peptide (GRP) receptors. Bombesin is a neuropeptide with a high affinity for these GRP receptors. The purpose of this study was to prepare and evaluate the characteristics of a new Freeze-dried kit, [6-hydrazinopyr...
متن کاملA lyophilized peptide radiopharmaceutical kit to target neurotensin receptor for tumor imaging
Introduction: Neurotensin (NT) is a tridecapeptide that binds specifically to neurotensin receptors. Several forms of cancer, including small cell lung cancer, colon, pancreatic and prostate carcinomas especially exocrine pancreatic carcinomas express receptors for neurotensin peptide. Radiolabeled neurotensin derivatives with a high affinity for these receptors might be used for scintigraphy. ...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
iranian journal of nuclear medicineناشر: tehran university of medical sciences
ISSN 1681-2824
دوره 23
شماره 1 2014
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023